rabbit polyclonal antibodies anti-gapdh sc-25778  (Santa Cruz Biotechnology)

Journal: PLoS ONE

Article Title: The role of vasodilator-stimulated phosphoprotein (VASP) in the control of hepatic gluconeogenic gene expression

doi: 10.1371/journal.pone.0215601

Figure Lengend Snippet: A . AML12 hepatocytes were treated with 8Br-cAMP (100μM) for a time period between 0–16 h, and both protein and RNA was harvested. Protein lysates were analyzed for p-VASP (Ser157) and VASP protein levels by Western blot (n = 3) p<0.05 time 0 vs. 1 h. B. Pck1 and G6p levels were determined by RT-PCR. Relative mRNA levels were normalized to Gapdh (n = 3) p<0.05 time 0 vs. 4h. C . C57BL6 male mice on chow diet were sacrificed either in the fed state or after 4 hours fast. Hepatic expression of p-VASP (Ser157) as measured by Western blot (n = 4 fast, n = 4 fed). D . Pck1 , G6p , Fbp1 by RT-PCR (n = 4 fast, n = 4 fed). *P<0.05 WT , wild type; IB, immunoblot.

Article Snippet: Anti-GAPDH (sc-25778) rabbit polyclonal antibody and anti CREB binding protein (CBP) (sc369) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction, Expressing